package ij.plugin.filter; import ij.*; import ij.gui.DialogListener; import ij.gui.GenericDialog; import ij.process.*; import java.awt.AWTEvent; import java.awt.Rectangle; /** This plug-in filter uses convolution with a Gaussian function for smoothing. * 'Radius' means the radius of decay to exp(-0.5) ~ 61%, i.e. the standard * deviation sigma of the Gaussian (this is the same as in Photoshop, but * different from the 'Gaussian Blur' in ImageJ versions before 1.38u, where * a value 2.5 times as much had to be entered. * - Like all convolution operations in ImageJ, it assumes that out-of-image * pixels have a value equal to the nearest edge pixel. This gives higher * weight to edge pixels than pixels inside the image, and higher weight * to corner pixels than non-corner pixels at the edge. Thus, when smoothing * with very high blur radius, the output will be dominated by the edge * pixels and especially the corner pixels (in the extreme case, with * a blur radius of e.g. 1e20, the image will be raplaced by the average * of the four corner pixels). * - For increased speed, except for small blur radii, the lines (rows or * columns of the image) are downscaled before convolution and upscaled * to their original length thereafter. * * Version 03-Jun-2007 M. Schmid with preview, progressBar stack-aware, * snapshot via snapshot flag; restricted range for resetOutOfRoi * * 20-Feb-2010 S. Saalfeld inner multi-threading * */ public class GaussianBlur implements ExtendedPlugInFilter, DialogListener { /** the standard deviation of the Gaussian*/ private static double sigma = 2.0; /** whether sigma is given in units corresponding to the pixel scale (not pixels)*/ private static boolean sigmaScaled = false; /** The flags specifying the capabilities and needs */ private int flags = DOES_ALL|SUPPORTS_MASKING|KEEP_PREVIEW; private ImagePlus imp; // The ImagePlus of the setup call, needed to get the spatial calibration private boolean hasScale = false; // whether the image has an x&y scale private int nPasses = 1; // The number of passes (filter directions * color channels * stack slices) private int nChannels = 1; // The number of color channels private int pass; // Current pass private boolean noProgress; // Do not show progress bar private boolean calledAsPlugin; /** Method to return types supported * @param arg unused * @param imp The ImagePlus, used to get the spatial calibration * @return Code describing supported formats etc. * (see ij.plugin.filter.PlugInFilter & ExtendedPlugInFilter) */ public int setup(String arg, ImagePlus imp) { this.imp = imp; if (imp!=null && imp.getRoi()!=null) { Rectangle roiRect = imp.getRoi().getBoundingRect(); if (roiRect.y > 0 || roiRect.y+roiRect.height < imp.getDimensions()[1]) flags |= SNAPSHOT; // snapshot for pixels above and/or below roi rectangle } return flags; } /** Ask the user for the parameters */ public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) { calledAsPlugin = true;; String options = Macro.getOptions(); boolean oldMacro = false; nChannels = imp.getProcessor().getNChannels(); if (options!=null) { if (options.indexOf("radius=") >= 0) { // ensure compatibility with old macros oldMacro = true; // specifying "radius=", not "sigma= Macro.setOptions(options.replaceAll("radius=", "sigma=")); } } GenericDialog gd = new GenericDialog(command); sigma = Math.abs(sigma); gd.addNumericField("Sigma (Radius):", sigma, 2); if (imp.getCalibration()!=null && !imp.getCalibration().getUnits().equals("pixels")) { hasScale = true; gd.addCheckbox("Scaled Units ("+imp.getCalibration().getUnits()+")", sigmaScaled); } else sigmaScaled = false; gd.addPreviewCheckbox(pfr); gd.addDialogListener(this); gd.showDialog(); // input by the user (or macro) happens here if (gd.wasCanceled()) return DONE; if (oldMacro) sigma /= 2.5; // for old macros, "radius" was 2.5 sigma IJ.register(this.getClass()); // protect static class variables (parameters) from garbage collection return IJ.setupDialog(imp, flags); // ask whether to process all slices of stack (if a stack) } /** Listener to modifications of the input fields of the dialog */ public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) { sigma = gd.getNextNumber(); if (sigma < 0 || gd.invalidNumber()) return false; if (hasScale) sigmaScaled = gd.getNextBoolean(); return true; } /** Set the number of passes of the blur1Direction method. If called by the * PlugInFilterRunner of ImageJ, an ImagePlus is known and conversion of RGB images * to float as well as the two filter directions are taken into account. * Otherwise, the caller should set nPasses to the number of 1-dimensional * filter operations required. */ public void setNPasses(int nPasses) { this.nPasses = 2 * nChannels * nPasses; pass = 0; } /** This method is invoked for each slice during execution * @param ip The image subject to filtering. It must have a valid snapshot if * the height of the roi is less than the full image height. */ public void run(ImageProcessor ip) { double sigmaX = sigmaScaled ? sigma/imp.getCalibration().pixelWidth : sigma; double sigmaY = sigmaScaled ? sigma/imp.getCalibration().pixelHeight : sigma; double accuracy = (ip instanceof ByteProcessor || ip instanceof ColorProcessor) ? 0.002 : 0.0002; Rectangle roi = ip.getRoi(); blurGaussian(ip, sigmaX, sigmaY, accuracy); } /** Gaussian Filtering of an ImageProcessor. This method is for compatibility with the * previous code (before 1.38r) and uses a low-accuracy kernel, only slightly better * than the previous ImageJ code. * The 'radius' in this call is different from the one used in ImageJ 1.38r and later. * Therefore, use blurGaussian(ip, sigma, sigma, accuracy), where 'sigma' is equivalent * to the 'sigma (radius)' of the Menu, and accuracy should be 0.02 unless better * accuracy is desired. */ @Deprecated public boolean blur(ImageProcessor ip, double radius) { Rectangle roi = ip.getRoi(); if (roi.height!=ip.getHeight() && ip.getMask()==null) ip.snapshot(); // a snapshot is needed for out-of-Rectangle pixels blurGaussian(ip, 0.4*radius, 0.4*radius, 0.01); return true; } /** Gaussian Filtering of an ImageProcessor * @param ip The ImageProcessor to be filtered. * @param sigma Standard deviation of the Gaussian (pixels) * * @see ij.process.ImageProcessor#blurGaussian(double) */ public void blurGaussian(ImageProcessor ip, double sigma) { double accuracy = (ip instanceof ByteProcessor||ip instanceof ColorProcessor)?0.002:0.0002; blurGaussian(ip, sigma, sigma, accuracy); } /** Gaussian Filtering of an ImageProcessor * @param ip The ImageProcessor to be filtered. * @param sigmaX Standard deviation of the Gaussian in x direction (pixels) * @param sigmaY Standard deviation of the Gaussian in y direction (pixels) * @param accuracy Accuracy of kernel, should not be above 0.02. Better (lower) * accuracy needs slightly more computing time. */ public void blurGaussian(ImageProcessor ip, double sigmaX, double sigmaY, double accuracy) { boolean hasRoi = ip.getRoi().height!=ip.getHeight() && sigmaX>0 && sigmaY>0; if (hasRoi && !calledAsPlugin) ip.snapshot(); if (nPasses<=1) nPasses = ip.getNChannels() * (sigmaX>0 && sigmaY>0 ? 2 : 1); FloatProcessor fp = null; for (int i=0; iand write the result into *0) blur1Direction(ip, sigmaX, accuracy, true, (int)Math.ceil(5*sigmaY)); if (Thread.currentThread().isInterrupted()) return; // interruption for new parameters during preview? if (sigmaY > 0) blur1Direction(ip, sigmaY, accuracy, false, 0); return; } /** Blur an image in one direction (x or y) by a Gaussian. * @param ip The Image with the original data where also the result will be stored * @param sigma Standard deviation of the Gaussian * @param accuracy Accuracy of kernel, should not be > 0.02 * @param xDirection True for bluring in x direction, false for y direction * @param extraLines Number of lines (parallel to the blurring direction) * below and above the roi bounds that should be processed. */ public void blur1Direction( final FloatProcessor ip, final double sigma, final double accuracy, final boolean xDirection, final int extraLines) { final int UPSCALE_K_RADIUS = 2; //number of pixels to add for upscaling final double MIN_DOWNSCALED_SIGMA = 4.; //minimum standard deviation in the downscaled image final float[] pixels = (float[])ip.getPixels(); final int width = ip.getWidth(); final int height = ip.getHeight(); final Rectangle roi = ip.getRoi(); final int length = xDirection ? width : height; //number of points per line (line can be a row or column) final int pointInc = xDirection ? 1 : width; //increment of the pixels array index to the next point in a line final int lineInc = xDirection ? width : 1; //increment of the pixels array index to the next line final int lineFromA = (xDirection ? roi.y : roi.x) - extraLines; //the first line to process final int lineFrom; if (lineFromA < 0) lineFrom = 0; else lineFrom = lineFromA; final int lineToA = (xDirection ? roi.y+roi.height : roi.x+roi.width) + extraLines; //the last line+1 to process final int lineTo; if (lineToA > (xDirection ? height:width)) lineTo = (xDirection ? height:width); else lineTo = lineToA; final int writeFrom = xDirection? roi.x : roi.y; //first point of a line that needs to be written final int writeTo = xDirection ? roi.x+roi.width : roi.y+roi.height; pass++; if (pass>nPasses) pass =1; final int numThreads = Math.min(Prefs.getThreads(), lineTo-lineFrom); final Thread[] lineThreads = new Thread[numThreads]; /* large radius (sigma): scale down, then convolve, then scale up */ final boolean doDownscaling = sigma > 2*MIN_DOWNSCALED_SIGMA + 0.5; final int reduceBy = doDownscaling ? //downscale by this factor Math.min((int)Math.floor(sigma/MIN_DOWNSCALED_SIGMA), length) : 1; /* Downscaling and upscaling blur the image a bit - we have to correct the standard * deviation for this: * Downscaling gives std devation sigma = 1/sqrt(3); upscale gives sigma = 1/2 (in downscaled pixels). * All sigma^2 values add to full sigma^2, which should be the desired value */ final double sigmaGauss = doDownscaling ? Math.sqrt(sigma*sigma/(reduceBy*reduceBy) - 1./3. - 1./4.) : sigma; final int maxLength = doDownscaling ? (length+reduceBy-1)/reduceBy + 2*(UPSCALE_K_RADIUS + 1) //downscaled line can't be longer : length; final float[][] gaussKernel = makeGaussianKernel(sigmaGauss, accuracy, maxLength); final int kRadius = gaussKernel[0].length*reduceBy; //Gaussian kernel radius after upscaling final int readFrom = (writeFrom-kRadius < 0) ? 0 : writeFrom-kRadius; //not including broadening by downscale&upscale final int readTo = (writeTo+kRadius > length) ? length : writeTo+kRadius; final int newLength = doDownscaling ? //line length for convolution (readTo-readFrom+reduceBy-1)/reduceBy + 2*(UPSCALE_K_RADIUS + 1) : length; final int unscaled0 = readFrom - (UPSCALE_K_RADIUS + 1)*reduceBy; //input point corresponding to cache index 0 //the following is relevant for upscaling only //IJ.log("reduce="+reduceBy+", newLength="+newLength+", unscaled0="+unscaled0+", sigmaG="+(float)sigmaGauss+", kRadius="+gaussKernel[0].length); final float[] downscaleKernel = doDownscaling ? makeDownscaleKernel(reduceBy) : null; final float[] upscaleKernel = doDownscaling ? makeUpscaleKernel(reduceBy) : null; for ( int t = 0; t < numThreads; ++t ) { final int ti = t; final float[] cache1 = new float[newLength]; //holds data before convolution (after downscaling, if any) final float[] cache2 = doDownscaling ? new float[newLength] : null; //holds data after convolution final Thread thread = new Thread( new Runnable() { final public void run() { /*try{*/ long lastTime = System.currentTimeMillis(); boolean canShowProgress = Thread.currentThread() == lineThreads[0]; int pixel0 = (lineFrom+ti)*lineInc; for (int line=lineFrom + ti; line 110) { if (canShowProgress) showProgress((double)(line-lineFrom)/(lineTo-lineFrom)); if (Thread.currentThread().isInterrupted()) return; // interruption for new parameters during preview? lastTime = time; } if (doDownscaling) { downscaleLine(pixels, cache1, downscaleKernel, reduceBy, pixel0, unscaled0, length, pointInc, newLength); convolveLine(cache1, cache2, gaussKernel, 0, newLength, 1, newLength-1, 0, 1); upscaleLine(cache2, pixels, upscaleKernel, reduceBy, pixel0, unscaled0, writeFrom, writeTo, pointInc); } else { int p = pixel0 + readFrom*pointInc; for (int i=readFrom; i reduceBy cache. * Input line pixel #unscaled0will correspond to output * line pixel # 0.unscaled0may be negative. Out-of-line * pixels of the input are replaced by the edge pixels. * @param pixels input array * @param cache output array * @param kernel downscale kernel, runs form -1.5 to +1.5 in downscaled coordinates * @param reduceBy downscaling factor * @param pixel0 index in pixels array corresponding to start of line or column * @param unscaled0 index in input line corresponding to output line index 0, May be negative. * @param length length of full input line or column * @param pointInc spacing of values in input array (1 for lines, image width for columns) * @param newLength length of downscaled data */ final static private void downscaleLine(final float[] pixels, final float[] cache, final float[] kernel, final int reduceBy, final int pixel0, final int unscaled0, final int length, final int pointInc, final int newLength) { int p = pixel0 + pointInc*(unscaled0-reduceBy*3/2); //pointer in pixels array final int pLast = pixel0 + pointInc*(length-1); for (int xout=-1; xout<=newLength; xout++) { float sum0 = 0, sum1 = 0, sum2 = 0; for (int x=0; xpLast ? pLast : p)]; sum0 += v * kernel[x+2*reduceBy]; sum1 += v * kernel[x+reduceBy]; sum2 += v * kernel[x]; } if (xout>0) cache[xout-1] += sum0; if (xout>=0 && xout pLast ? pLast : pp)]; v += kernel[x+reduceBy] * pixels[p pLast ? pLast : p)]; pp = p+pointInc*reduceBy; v += kernel[x+2*reduceBy] * pixels[pp pLast ? pLast : pp)]; } cache[xout] = v; } }*/ /* Create a kernel for downscaling. The kernel function preserves * norm and 1st moment (i.e., position) and has fixed 2nd moment, * (in contrast to linear interpolation). * In scaled space, the length of the kernel runs from -1.5 to +1.5, * and the standard deviation is 1/2. * Array index corresponding to the kernel center is * unitLength*3/2 */ final static private float[] makeDownscaleKernel (final int unitLength) { final int mid = unitLength*3/2; final float[] kernel = new float[3*unitLength]; for (int i=0; i<=unitLength/2; i++) { final double x = i/(double)unitLength; final float v = (float)((0.75-x*x)/unitLength); kernel[mid-i] = v; kernel[mid+i] = v; } for (int i=unitLength/2+1; i<(unitLength*3+1)/2; i++) { final double x = i/(double)unitLength; final float v = (float)((0.125 + 0.5*(x-1)*(x-2))/unitLength); kernel[mid-i] = v; kernel[mid+i] = v; } return kernel; } /** Scale a line up by factor reduceByand write as a row * or column (or part thereof) to the pixels array of a FloatProcessor. */ final static private void upscaleLine (final float[] cache, final float[] pixels, final float[] kernel, final int reduceBy, final int pixel0, final int unscaled0, final int writeFrom, final int writeTo, final int pointInc) { int p = pixel0 + pointInc*writeFrom; for (int xout = writeFrom; xout < writeTo; xout++, p+=pointInc) { final int xin = (xout-unscaled0+reduceBy-1)/reduceBy; //the corresponding point in the cache (if exact) or the one above final int x = reduceBy - 1 - (xout-unscaled0+reduceBy-1)%reduceBy; pixels[p] = cache[xin-2]*kernel[x] + cache[xin-1]*kernel[x+reduceBy] + cache[xin]*kernel[x+2*reduceBy] + cache[xin+1]*kernel[x+3*reduceBy]; } } /** Create a kernel for upscaling. The kernel function is a convolution * of four unit squares, i.e., four uniform kernels with value +1 * from -0.5 to +0.5 (in downscaled coordinates). The second derivative * of this kernel is smooth, the third is not. Its standard deviation * is 1/sqrt(3) in downscaled cordinates. * The kernel runs from [-2 to +2[, corresponding to array index * 0 ... 4*unitLength (whereby the last point is not in the array any more). */ final static private float[] makeUpscaleKernel (final int unitLength) { final float[] kernel = new float[4*unitLength]; final int mid = 2*unitLength; kernel[0] = 0; for (int i=0; iwriteFrom-kernel.length or 0. * @param readTo Last array element+1 of the line that must be read. * writeTo+kernel.lengthorinput.length* @param writeFrom Index of the first point in the line that should be written * @param writeTo Index+1 of the last point in the line that should be written * @param point0 Array index of first element of the 'line' in pixels (i.e., lineNumber * lineInc) * @param pointInc Increment of the pixels array index to the next point (for an ImageProcessor, * it should be1for a row,widthfor a column) */ final static private void convolveLine( final float[] input, final float[] pixels, final float[][] kernel, final int readFrom, final int readTo, final int writeFrom, final int writeTo, final int point0, final int pointInc) { final int length = input.length; final float first = input[0]; //out-of-edge pixels are replaced by nearest edge pixels final float last = input[length-1]; final float[] kern = kernel[0]; //the kernel itself final float kern0 = kern[0]; final float[] kernSum = kernel[1]; //the running sum over the kernel final int kRadius = kern.length; final int firstPart = kRadius < length ? kRadius : length; int p = point0 + writeFrom*pointInc; int i = writeFrom; for (; ilength) result += kernSum[length-i-1]*last; for (int k=1; k = 0) v += input[i-k]; if (i+k = length float result = input[i]*kern0; if (i =length) result += kernSum[length-i-1]*last; for (int k=1; k = 0) v += input[i-k]; if (i+k n, including non-calculated values in case the kernel * size is limited by maxRadius. */ public float[][] makeGaussianKernel(final double sigma, final double accuracy, int maxRadius) { int kRadius = (int)Math.ceil(sigma*Math.sqrt(-2*Math.log(accuracy)))+1; if (maxRadius < 50) maxRadius = 50; // too small maxRadius would result in inaccurate sum. if (kRadius > maxRadius) kRadius = maxRadius; float[][] kernel = new float[2][kRadius]; for (int i=0; i3) { // edge correction double sqrtSlope = Double.MAX_VALUE; int r = kRadius; while (r > kRadius/2) { r--; double a = Math.sqrt(kernel[0][r])/(kRadius-r); if (a < sqrtSlope) sqrtSlope = a; else break; } for (int r1 = r+2; r1 < kRadius; r1++) kernel[0][r1] = (float)((kRadius-r1)*(kRadius-r1)*sqrtSlope*sqrtSlope); } double sum; // sum over all kernel elements for normalization if (kRadius < maxRadius) { sum = kernel[0][0]; for (int i=1; i Ads help maintain this website.