*/ void makeInterpolationArrays(int[] smallIndices, float[] weights, int length, int smallLength, int shrinkFactor) { for (int i=0; ipackage ij.plugin.filter; import ij.*; import ij.gui.*; import ij.process.*; import ij.measure.*; import ij.util.Tools; import java.awt.*; /** Implements ImageJ's Subtract Background command. Based on the concept of the rolling ball algorithm described in Stanley Sternberg's article, "Biomedical Image Processing", IEEE Computer, January 1983. Imagine that the 2D grayscale image has a third (height) dimension by the image value at every point in the image, creating a surface. A ball of given radius is rolled over the bottom side of this surface; the hull of the volume reachable by the ball is the background. With "Sliding Parabvoloid", the rolling ball is replaced by a sliding paraboloid of rotation with the same curvature at its apex as a ball of a given radius. A paraboloid has the advantage that suitable paraboloids can be found for any image values, even if the pixel values are much larger than a typical object size (in pixels). The paraboloid of rotation is approximated as parabolae in 4 directions: x, y and the two 45-degree directions. Lines of the image in these directions are processed by sliding a parabola against them. Obtaining the hull needs the parabola for a given direction to be applied multiple times (after doing the other directions); in this respect the current code is a compromise between accuracy and speed. For noise rejection, with the sliding paraboloid algorithm, a 3x3 maximum of the background is applied. With both, rolling ball and sliding paraboloid, the image used for calculating the background is slightly smoothened (3x3 average). This can result in negative values after background subtraction. This preprocessing can be disabled. In the sliding paraboloid algorithm, additional code has been added to avoid subtracting corner objects as a background (note that a paraboloid or ball would always touch the 4 corner pixels and thus make them background pixels). This code assumes that corner particles reach less than 1/4 of the image size into the image. Rolling ball code based on the NIH Image Pascal version by Michael Castle and Janice Keller of the University of Michigan Mental Health Research Institute. Sliding Paraboloid by Michael Schmid, 2007. Version 10-Jan-2008 */ public class BackgroundSubtracter implements ExtendedPlugInFilter, DialogListener { /* parameters from the dialog: */ private static double staticRadius = 50; // default rolling ball radius private static boolean staticLightBackground = Prefs.get("bs.background", true); private static boolean staticSeparateColors; // whether to create a separate background for each color channel private static boolean staticCreateBackground; // don't subtract background (e.g., for processing the background before subtracting) private static boolean staticUseParaboloid; // use "Sliding Paraboloid" instead of rolling ball algorithm private static boolean staticDoPresmooth = true; // smoothen the image before creating the background private double radius = staticRadius; private boolean lightBackground = staticLightBackground; private boolean separateColors = staticSeparateColors; private boolean createBackground = staticCreateBackground; private boolean useParaboloid = staticUseParaboloid; private boolean doPresmooth = staticDoPresmooth; /* more class variables */ private boolean isRGB; // whether we have an RGB image private boolean previewing; private final static int MAXIMUM = 0, MEAN = 1; //filter types of filter3x3 private final static int X_DIRECTION = 0, Y_DIRECTION = 1, DIAGONAL_1A = 2, DIAGONAL_1B = 3, DIAGONAL_2A = 4, DIAGONAL_2B = 5; //filter directions private final static int DIRECTION_PASSES = 9; //number of passes for different directions private int nPasses = DIRECTION_PASSES; private int pass; private int flags = DOES_ALL|FINAL_PROCESSING|KEEP_PREVIEW|PARALLELIZE_STACKS; private boolean calledAsPlugin; public int setup(String arg, ImagePlus imp) { if (arg.equals("final")) { imp.getProcessor().resetMinAndMax(); return DONE; } else return flags; } public int showDialog(ImagePlus imp, String command, PlugInFilterRunner pfr) { isRGB = imp.getProcessor() instanceof ColorProcessor; calledAsPlugin = true; String options = Macro.getOptions(); if (options!=null) { //macro Macro.setOptions(options.replaceAll("white", "light")); radius = 50; lightBackground = false; separateColors = false; createBackground = false; useParaboloid = false; doPresmooth = true; } GenericDialog gd = new GenericDialog(command); gd.addNumericField("Rolling ball radius:", radius, 1, 6, "pixels"); gd.addCheckbox("Light background", lightBackground); if (isRGB) gd.addCheckbox("Separate colors", separateColors); gd.addCheckbox("Create background (don't subtract)", createBackground); gd.addCheckbox("Sliding paraboloid", useParaboloid); gd.addCheckbox("Disable smoothing", !doPresmooth); gd.addPreviewCheckbox(pfr); gd.addDialogListener(this); previewing = true; gd.addHelp(IJ.URL+"/docs/menus/process.html#background"); gd.showDialog(); previewing = false; if (gd.wasCanceled()) return DONE; IJ.register(this.getClass()); //protect static class variables (filter parameters) from garbage collection if ((imp.getProcessor() instanceof FloatProcessor) && !createBackground) flags |= SNAPSHOT; //FloatProcessors need the original to subtract it from the background if (options==null) { // not a macro staticRadius = radius; staticLightBackground = lightBackground; staticSeparateColors = separateColors; staticCreateBackground = createBackground; staticUseParaboloid = useParaboloid; staticDoPresmooth = doPresmooth; Prefs.set("bs.background", lightBackground); } return IJ.setupDialog(imp, flags); //ask whether to process all slices of stack (if a stack) } public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) { radius = gd.getNextNumber(); if (radius <= 0.0001 || gd.invalidNumber()) return false; lightBackground = gd.getNextBoolean(); if (isRGB) separateColors = gd.getNextBoolean(); createBackground = gd.getNextBoolean(); useParaboloid = gd.getNextBoolean(); doPresmooth = !gd.getNextBoolean(); return true; } /** Background for any image type */ public void run(ImageProcessor ip) { if (isRGB && !separateColors) rollingBallBrightnessBackground((ColorProcessor)ip, radius, createBackground, lightBackground, useParaboloid, doPresmooth, true); else rollingBallBackground(ip, radius, createBackground, lightBackground, useParaboloid, doPresmooth, true); if (previewing && (ip instanceof FloatProcessor || ip instanceof ShortProcessor)) { ip.resetMinAndMax(); } } /** Depracated. For compatibility with previous ImageJ versions */ public void subtractRGBBackround(ColorProcessor ip, int ballRadius) { rollingBallBrightnessBackground(ip, (double)ballRadius, false, lightBackground, false, true, true); } /** Depracated. For compatibility with previous ImageJ versions */ public void subtractBackround(ImageProcessor ip, int ballRadius) { rollingBallBackground(ip, (double)ballRadius, false, lightBackground, false, true, true); } /** Create or subtract a background, based on the brightness of an RGB image (keeping * the hue of each pixel unchanged) * @param ip The RGB image. On output, it will become the background-subtracted image or * the background (depending on. Will usually remain * in the CPU cache and may therefore speed up the code. * @param nextPoint Work array. Will hold the index of the next point with sufficient local * curvature to get touched by the parabola. * @param correctedEdges Should be a 2-element array used for output or null. * @return The correctedEdges array (if non-null on input) with the two estimated * edge pixel values corrected for edge particles. */ static float[] lineSlideParabola(float[] pixels, int start, int inc, int length, float coeff2, float[] cache, int[] nextPoint, float[] correctedEdges) { float minValue = Float.MAX_VALUE; int lastpoint = 0; int firstCorner = length-1; // the first point except the edge that is touched int lastCorner = 0; // the last point except the edge that is touched float vPrevious1 = 0f; float vPrevious2 = 0f; float curvatureTest = 1.999f*coeff2; //not 2: numeric scatter of 2nd derivative /* copy data to cache, determine the minimum, and find points with local curvature such * that the parabola can touch them - only these need to be examined futher on */ for (int i=0, p=start; icreateBackground). * @param radius Radius of the rolling ball creating the background (actually a * paraboloid of rotation with the same curvature) * @param createBackground Whether to create a background, not to subtract it. * @param lightBackground Whether the image has a light background. * @param doPresmooth Whether the image should be smoothened (3x3 mean) before creating * the background. With smoothing, the background will not necessarily * be below the image data. * @param correctCorners Whether the algorithm should try to detect corner particles to avoid * subtracting them as a background. */ public void rollingBallBrightnessBackground(ColorProcessor ip, double radius, boolean createBackground, boolean lightBackground, boolean useParaboloid, boolean doPresmooth, boolean correctCorners) { int width = ip.getWidth(); int height = ip.getHeight(); byte[] H = new byte[width*height]; byte[] S = new byte[width*height]; byte[] B = new byte[width*height]; ip.getHSB(H, S, B); ByteProcessor bp = new ByteProcessor(width, height, B, null); rollingBallBackground(bp, radius, createBackground, lightBackground, useParaboloid, doPresmooth, correctCorners); ip.setHSB(H, S, (byte[])bp.getPixels()); } /** Create or subtract a background, works for all image types. For RGB images, the * background is subtracted from each channel separately * @param ip The image. On output, it will become the background-subtracted image or * the background (depending oncreateBackground). * @param radius Radius of the rolling ball creating the background (actually a * paraboloid of rotation with the same curvature) * @param createBackground Whether to create a background, not to subtract it. * @param lightBackground Whether the image has a light background. * @param useParaboloid Whether to use the "sliding paraboloid" algorithm. * @param doPresmooth Whether the image should be smoothened (3x3 mean) before creating * the background. With smoothing, the background will not necessarily * be below the image data. * @param correctCorners Whether the algorithm should try to detect corner particles to avoid * subtracting them as a background. */ public void rollingBallBackground(ImageProcessor ip, double radius, boolean createBackground, boolean lightBackground, boolean useParaboloid, boolean doPresmooth, boolean correctCorners) { boolean invertedLut = ip.isInvertedLut(); boolean invert = (invertedLut && !lightBackground) || (!invertedLut && lightBackground); RollingBall ball = null; if (!useParaboloid) ball = new RollingBall(radius); FloatProcessor fp = null; for (int channelNumber=0; channelNumber65535f) value = 65535f; pixels[p] = (short)(value); } } else if (ip instanceof ByteProcessor) { float offset = invert ? 255.5f : 0.5f; //includes 0.5 for rounding when converting float to byte byte[] pixels = (byte[])ip.getPixels(); for (int p=0; p 255f) value = 255f; pixels[p] = (byte)(value); } } else if (ip instanceof ColorProcessor) { float offset = invert ? 255.5f : 0.5f; int[] pixels = (int[])ip.getPixels(); int shift = 16 - 8*channelNumber; int byteMask = 255< >shift) - bgPixels[p] + offset; if (value<0f) value = 0f; if (value>255f) value = 255f; pixels[p] = (pxl&resetMask) | ((int)value< length = 2 && vPrevious1+vPrevious1-vPrevious2-v < curvatureTest) { nextPoint[lastpoint] = i-1; // point i-1 may be touched lastpoint = i-1; } vPrevious2 = vPrevious1; vPrevious1 = v; } nextPoint[lastpoint] = length-1; nextPoint[length-1] = Integer.MAX_VALUE;// breaks the search loop int i1 = 0; // i1 and i2 will be the two points where the parabola touches while (i1 0) searchTo = maxSearch; } } if (i1 == 0) firstCorner = i2; if (i2 == length-1) lastCorner = i1; /* interpolate between the two points where the parabola touches: */ for (int j=i1+1, p=start+j*inc; j = length) firstCorner = 0; // edge particles must be < 1/4 image size if (4*(length - 1 - lastCorner) >= length) lastCorner = length - 1; float v1 = cache[firstCorner]; float v2 = cache[lastCorner]; float slope = (v2-v1)/(lastCorner-firstCorner); // of the line through the two outermost non-edge touching points float value0 = v1 - slope * firstCorner; // offset of this line float coeff6 = 0; // coefficient of 6th order polynomial float mid = 0.5f * (lastCorner + firstCorner); for (int i=(length+2)/3; i<=(2*length)/3; i++) {// compare with mid-image pixels to detect vignetting float dx = (i-mid)*2f/(lastCorner-firstCorner); float poly6 = dx*dx*dx*dx*dx*dx - 1f; // the 6th order polynomial, zero at firstCorner and lastCorner if (cache[i] < value0 + slope*i + coeff6*poly6) { coeff6 = -(value0 + slope*i - cache[i])/poly6; } } float dx = (firstCorner-mid)*2f/(lastCorner-firstCorner); correctedEdges[0] = value0 + coeff6*(dx*dx*dx*dx*dx*dx - 1f) + coeff2*firstCorner*firstCorner; dx = (lastCorner-mid)*2f/(lastCorner-firstCorner); correctedEdges[1] = value0 + (length-1)*slope + coeff6*(dx*dx*dx*dx*dx*dx - 1f) + coeff2*(length-1-lastCorner)*(length-1-lastCorner); } return correctedEdges; } //void lineSlideParabola /** Detect corner particles and adjust corner pixels if a particle is there. * Analyzing the directions parallel to the edges and the diagonals, we * average over the 3 correction values (found for the 3 directions) */ void correctCorners(FloatProcessor fp, float coeff2, float[] cache, int[] nextPoint) { int width = fp.getWidth(); int height = fp.getHeight(); float[] pixels = (float[])fp.getPixels(); float[] corners = new float[4]; //(0,0); (xmax,0); (ymax,0); (xmax,ymax) float[] correctedEdges = new float[2]; correctedEdges = lineSlideParabola(pixels, 0, 1, width, coeff2, cache, nextPoint, correctedEdges); corners[0] = correctedEdges[0]; corners[1] = correctedEdges[1]; correctedEdges = lineSlideParabola(pixels, (height-1)*width, 1, width, coeff2, cache, nextPoint, correctedEdges); corners[2] = correctedEdges[0]; corners[3] = correctedEdges[1]; correctedEdges = lineSlideParabola(pixels, 0, width, height, coeff2, cache, nextPoint, correctedEdges); corners[0] += correctedEdges[0]; corners[2] += correctedEdges[1]; correctedEdges = lineSlideParabola(pixels, width-1, width, height, coeff2, cache, nextPoint, correctedEdges); corners[1] += correctedEdges[0]; corners[3] += correctedEdges[1]; int diagLength = Math.min(width,height); //length of a 45-degree line from a corner float coeff2diag = 2 * coeff2; correctedEdges = lineSlideParabola(pixels, 0, 1+width, diagLength, coeff2diag, cache, nextPoint, correctedEdges); corners[0] += correctedEdges[0]; correctedEdges = lineSlideParabola(pixels, width-1, -1+width, diagLength, coeff2diag, cache, nextPoint, correctedEdges); corners[1] += correctedEdges[0]; correctedEdges = lineSlideParabola(pixels, (height-1)*width, 1-width, diagLength, coeff2diag, cache, nextPoint, correctedEdges); corners[2] += correctedEdges[0]; correctedEdges = lineSlideParabola(pixels, width*height-1, -1-width, diagLength, coeff2diag, cache, nextPoint, correctedEdges); corners[3] += correctedEdges[0]; if (pixels[0] > corners[0]/3) pixels[0] = corners[0]/3; if (pixels[width-1] > corners[1]/3) pixels[width-1] = corners[1]/3; if (pixels[(height-1)*width] > corners[2]/3) pixels[(height-1)*width] = corners[2]/3; if (pixels[width*height-1] > corners[3]/3) pixels[width*height-1] = corners[3]/3; //new ImagePlus("corner corrected",fp.duplicate()).show(); } //void correctCorners // R O L L B A L L S E C T I O N /** Create background for a float image by rolling a ball over * the image. */ void rollingBallFloatBackground(FloatProcessor fp, float radius, boolean invert, boolean doPresmooth, RollingBall ball) { float[] pixels = (float[])fp.getPixels(); //this will become the background boolean shrink = ball.shrinkFactor >1; showProgress(0.0); if (invert) for (int i=0; i 100) { lastTime = time; if (thread.isInterrupted()) return; showProgress(0.1+0.8*y/(height+ballWidth)); } int nextLineToWriteInCache = (y+radius)%ballWidth; int nextLineToRead = y + radius; //line of the input not touched yet if (nextLineToRead =height) yend = height-1; for (int x=-radius; x =width) xend = width-1; for (int yp=y0, yBall=yBall0; yp<=yend; yp++,yBall++) { //for all points inside the ball int cachePointer = (yp%ballWidth)*width+x0; for (int xp=x0, bp=xBall0+yBall*ballWidth; xp<=xend; xp++, cachePointer++, bp++) { float zReduced = cache[cachePointer] - zBall[bp]; if (z > zReduced) //does this point imply a greater height? z = zReduced; } } for (int yp=y0, yBall=yBall0; yp<=yend; yp++,yBall++) //raise pixels to ball surface for (int xp=x0, p=xp+yp*width, bp=xBall0+yBall*ballWidth; xp<=xend; xp++, p++, bp++) { float zMin = z + zBall[bp]; if (pixels[p] < zMin) pixels[p] = zMin; } // if (x>=0&&y>=0&&x line0 line0 = line1; line1 = swap; //keep the other array for filling with new data ySmallLine0++; int sYPointer = (ySmallIndices[y]+1)*smallWidth; //points to line0 + 1 in smallImage for (int x=0; x line1 line1[x] = sPixels[sYPointer+xSmallIndices[x]] * xWeights[x] + sPixels[sYPointer+xSmallIndices[x]+1] * (1f - xWeights[x]); } float weight = yWeights[y]; for (int x=0, p=y*width; x Example for shrinkFactor = 4: small image pixel number | 0 | 1 | 2 | ... full image pixel number | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |10 |11 | ... smallIndex for interpolation(0) | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | ... (0) Note: This is smallIndex for the left pixel; for the right pixel used for interpolation it is higher by one
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