package ij.plugin;
import ij.*;
import ij.process.*;
import ij.gui.*;
import ij.measure.*;
import ij.util.Tools;
import java.awt.*;
import java.awt.event.*;
import java.util.*;
/** Implements the Image/Stacks/Reslice command. Known shortcomings:
for FREELINE or POLYLINE ROI, spatial calibration is ignored:
the image is sampled at constant _pixel_ increments (distance 1), so
(if y/x aspect ratio != 1 in source image) one dimension in the output is not
homogeneous (i.e. pixelWidth not the same everywhere).
*/
public class Slicer implements PlugIn, TextListener, ItemListener {
private static final String[] starts = {"Top", "Left", "Bottom", "Right"};
private static String startAtS = starts[0];
private static boolean rotateS;
private static boolean flipS;
private static int sliceCountS = 1;
private String startAt = starts[0];
private boolean rotate;
private boolean flip;
private int sliceCount = 1;
private boolean nointerpolate = Prefs.avoidResliceInterpolation;
private double inputZSpacing = 1.0;
private double outputZSpacing = 1.0;
private int outputSlices = 1;
private boolean noRoi;
private boolean rgb, notFloat;
private Vector fields, checkboxes;
private Label message;
private ImagePlus imp;
private double gx1, gy1, gx2, gy2, gLength;
// Variables used by getIrregularProfile and doIrregularSetup
private int n;
private double[] x;
private double[] y;
private int xbase;
private int ybase;
private double length;
private double[] segmentLengths;
private double[] dx;
private double[] dy;
public void run(String arg) {
imp = WindowManager.getCurrentImage();
if (imp==null) {
IJ.noImage();
return;
}
int stackSize = imp.getStackSize();
Roi roi = imp.getRoi();
int roiType = roi!=null?roi.getType():0;
// stack required except for ROI = none or RECT
if (stackSize<2 && roi!=null && roiType!=Roi.RECTANGLE) {
IJ.error("Reslice...", "Stack required");
return;
}
// permissible ROI types: none,RECT,*LINE
if (roi!=null && roiType!=Roi.RECTANGLE && roiType!=Roi.LINE && roiType!=Roi.POLYLINE && roiType!=Roi.FREELINE) {
IJ.error("Reslice...", "Line or rectangular selection required");
return;
}
if (!showDialog(imp))
return;
long startTime = System.currentTimeMillis();
ImagePlus imp2 = null;
rgb = imp.getType()==ImagePlus.COLOR_RGB;
notFloat = !rgb && imp.getType()!=ImagePlus.GRAY32;
if (imp.isHyperStack())
imp2 = resliceHyperstack(imp);
else
imp2 = reslice(imp);
if (imp2==null)
return;
ImageProcessor ip = imp.getProcessor();
double min = ip.getMin();
double max = ip.getMax();
if (!rgb) imp2.getProcessor().setMinAndMax(min, max);
imp2.show();
if (noRoi)
imp.deleteRoi();
else
imp.draw();
IJ.showStatus(IJ.d2s(((System.currentTimeMillis()-startTime)/1000.0),2)+" seconds");
}
public ImagePlus reslice(ImagePlus imp) {
ImagePlus imp2;
Roi roi = imp.getRoi();
int roiType = roi!=null?roi.getType():0;
Calibration origCal = imp.getCalibration();
boolean globalCalibration = false;
if (nointerpolate) {// temporarily clear spatial calibration
globalCalibration = imp.getGlobalCalibration()!=null;
imp.setGlobalCalibration(null);
Calibration tmpCal = origCal.copy();
tmpCal.pixelWidth = 1.0;
tmpCal.pixelHeight = 1.0;
tmpCal.pixelDepth = 1.0;
imp.setCalibration(tmpCal);
inputZSpacing = 1.0;
if (roiType!=Roi.LINE)
outputZSpacing = 1.0;
}
double zSpacing = inputZSpacing/imp.getCalibration().pixelWidth;
if (roi==null || roiType==Roi.RECTANGLE || roiType==Roi.LINE) {
imp2 = resliceRectOrLine(imp);
} else {// we assert roiType==Roi.POLYLINE || roiType==Roi.FREELINE
String status = imp.getStack().isVirtual()?"":null;
IJ.showStatus("Reslice...");
ImageProcessor ip2 = getSlice(imp, 0.0, 0.0, 0.0, 0.0, status);
imp2 = new ImagePlus("Reslice of "+imp.getShortTitle(), ip2);
}
if (nointerpolate) { // restore calibration
if (globalCalibration)
imp.setGlobalCalibration(origCal);
imp.setCalibration(origCal);
}
// create Calibration for new stack
// start from previous cal and swap appropriate fields
boolean horizontal = false;
boolean vertical = false;
if (roi==null || roiType==Roi.RECTANGLE) {
if (startAt.equals(starts[0]) || startAt.equals(starts[2]))
horizontal = true;
else
vertical = true;
}
if (roi!=null && roiType==Roi.LINE) {
Line l = (Line)roi;
horizontal = (l.y2-l.y1)==0;
vertical = (l.x2-l.x1)==0;
}
if (imp2==null) return null;
imp2.setCalibration(imp.getCalibration());
Calibration cal = imp2.getCalibration();
if (horizontal) {
cal.pixelWidth = origCal.pixelWidth;
cal.pixelHeight = origCal.pixelDepth/zSpacing;
cal.pixelDepth = origCal.pixelHeight*outputZSpacing;
} else if (vertical) {
cal.pixelWidth = origCal.pixelHeight;
cal.pixelHeight = origCal.pixelDepth/zSpacing;
//cal.pixelWidth = origCal.pixelDepth/zSpacing;
//cal.pixelHeight = origCal.pixelHeight;
cal.pixelDepth = origCal.pixelWidth*outputZSpacing;;
} else { // oblique line, polyLine or freeline
if (origCal.pixelHeight==origCal.pixelWidth) {
cal.pixelWidth = origCal.pixelWidth;
cal.pixelHeight=origCal.pixelDepth/zSpacing;
cal.pixelDepth = origCal.pixelWidth*outputZSpacing;
} else {
cal.pixelWidth = cal.pixelHeight=cal.pixelDepth=1.0;
cal.setUnit("pixel");
}
}
double tmp;
if (rotate) {// if rotated flip X and Y
tmp = cal.pixelWidth;
cal.pixelWidth = cal.pixelHeight;
cal.pixelHeight = tmp;
}
return imp2;
}
ImagePlus resliceHyperstack(ImagePlus imp) {
int channels = imp.getNChannels();
int slices = imp.getNSlices();
int frames = imp.getNFrames();
if (slices==1)
return resliceTimeLapseHyperstack(imp);
int c1 = imp.getChannel();
int z1 = imp.getSlice();
int t1 = imp.getFrame();
int width = imp.getWidth();
int height = imp.getHeight();
ImagePlus imp2 = null;
ImageStack stack2 = null;
Roi roi = imp.getRoi();
for (int t=1; t<=frames; t++) {
for (int c=1; c<=channels; c++) {
ImageStack tmp1Stack = new ImageStack(width, height);
for (int z=1; z<=slices; z++) {
imp.setPositionWithoutUpdate(c, z, t);
tmp1Stack.addSlice(null, imp.getProcessor());
}
ImagePlus tmp1 = new ImagePlus("tmp", tmp1Stack);
tmp1.setCalibration(imp.getCalibration());
tmp1.setRoi(roi);
ImagePlus tmp2 = reslice(tmp1);
int slices2 = tmp2.getStackSize();
if (imp2==null) {
imp2 = tmp2.createHyperStack("Reslice of "+imp.getTitle(), channels, slices2, frames, tmp2.getBitDepth());
stack2 = imp2.getStack();
}
ImageStack tmp2Stack = tmp2.getStack();
for (int z=1; z<=slices2; z++) {
imp.setPositionWithoutUpdate(c, z, t);
int n2 = imp2.getStackIndex(c, z, t);
stack2.setPixels(tmp2Stack.getPixels(z), n2);
}
}
}
imp.setPosition(c1, z1, t1);
if (channels>1 && imp.isComposite()) {
imp2 = new CompositeImage(imp2, ((CompositeImage)imp).getMode());
((CompositeImage)imp2).copyLuts(imp);
}
return imp2;
}
ImagePlus resliceTimeLapseHyperstack(ImagePlus imp) {
int channels = imp.getNChannels();
int frames = imp.getNFrames();
int c1 = imp.getChannel();
int t1 = imp.getFrame();
int width = imp.getWidth();
int height = imp.getHeight();
ImagePlus imp2 = null;
ImageStack stack2 = null;
Roi roi = imp.getRoi();
int z = 1;
for (int c=1; c<=channels; c++) {
ImageStack tmp1Stack = new ImageStack(width, height);
for (int t=1; t<=frames; t++) {
imp.setPositionWithoutUpdate(c, z, t);
tmp1Stack.addSlice(null, imp.getProcessor());
}
ImagePlus tmp1 = new ImagePlus("tmp", tmp1Stack);
tmp1.setCalibration(imp.getCalibration());
tmp1.setRoi(roi);
ImagePlus tmp2 = reslice(tmp1);
int frames2 = tmp2.getStackSize();
if (imp2==null) {
imp2 = tmp2.createHyperStack("Reslice of "+imp.getTitle(), channels, 1, frames2, tmp2.getBitDepth());
stack2 = imp2.getStack();
}
ImageStack tmp2Stack = tmp2.getStack();
for (int t=1; t<=frames2; t++) {
imp.setPositionWithoutUpdate(c, z, t);
int n2 = imp2.getStackIndex(c, z, t);
stack2.setPixels(tmp2Stack.getPixels(z), n2);
}
}
imp.setPosition(c1, 1, t1);
if (channels>1 && imp.isComposite()) {
imp2 = new CompositeImage(imp2, ((CompositeImage)imp).getMode());
((CompositeImage)imp2).copyLuts(imp);
}
return imp2;
}
boolean showDialog(ImagePlus imp) {
Calibration cal = imp.getCalibration();
if (cal.pixelDepth<0.0)
cal.pixelDepth = -cal.pixelDepth;
String units = cal.getUnits();
if (cal.pixelWidth==0.0)
cal.pixelWidth = 1.0;
inputZSpacing = cal.pixelDepth;
double outputSpacing = cal.pixelDepth;
Roi roi = imp.getRoi();
boolean line = roi!=null && roi.getType()==Roi.LINE;
if (line) saveLineInfo(roi);
String macroOptions = Macro.getOptions();
boolean macroRunning = macroOptions!=null;
if (macroRunning) {
if (macroOptions.indexOf("input=")!=-1)
macroOptions = macroOptions.replaceAll("slice=", "slice_count=");
macroOptions = macroOptions.replaceAll("slice=", "output=");
Macro.setOptions(macroOptions);
nointerpolate = false;
} else {
startAt = startAtS;
rotate = rotateS;
flip = flipS;
sliceCount = sliceCountS;
}
GenericDialog gd = new GenericDialog("Reslice");
gd.addNumericField("Output spacing ("+units+"):", outputSpacing, 3);
if (line) {
if (!IJ.isMacro()) outputSlices=sliceCount;
gd.addNumericField("Slice_count:", outputSlices, 0);
} else
gd.addChoice("Start at:", starts, startAt);
gd.addCheckbox("Flip vertically", flip);
gd.addCheckbox("Rotate 90 degrees", rotate);
gd.addCheckbox("Avoid interpolation", nointerpolate);
gd.setInsets(0, 32, 0);
gd.addMessage("(use 1 pixel spacing)");
gd.setInsets(15, 0, 0);
gd.addMessage("Voxel size: "+d2s(cal.pixelWidth)+"x"+d2s(cal.pixelHeight)
+"x"+d2s(cal.pixelDepth)+" "+cal.getUnit());
gd.setInsets(5, 0, 0);
gd.addMessage("Output size: "+getSize(cal.pixelDepth,outputSpacing,outputSlices)+" ");
fields = gd.getNumericFields();
if (!macroRunning) {
for (int i=0; iProperties correct?.");
return null;
}
boolean virtualStack = imp.getStack().isVirtual();
String status = null;
ImagePlus imp2 = null;
ImageStack stack2 = null;
boolean isStack = imp.getStackSize()>1;
IJ.resetEscape();
for (int i=0; i1?(i+1)+"/"+outputSlices+", ":"";
ImageProcessor ip = getSlice(imp, x1, y1, x2, y2, status);
//IJ.log(i+" "+x1+" "+y1+" "+x2+" "+y2+" "+ip);
if (isStack) drawLine(x1, y1, x2, y2, imp);
if (stack2==null) {
stack2 = createOutputStack(imp, ip);
if (stack2==null || stack2.getSize()0) makePolygon(count, outSpacing);
}
String size = getSize(inputZSpacing, outSpacing, count);
message.setText("Output Size: "+size);
}
String getSize(double inSpacing, double outSpacing, int count) {
int size = getOutputStackSize(inSpacing, outSpacing, count);
int mem = getAvailableMemory();
String available = mem!=-1?" ("+mem+"MB free)":"";
if (message!=null)
message.setForeground(mem!=-1&&size>mem?Color.red:Color.black);
if (size>0)
return size+"MB"+available;
else
return "<1MB"+available;
}
void makePolygon(int count, double outSpacing) {
int[] x = new int[4];
int[] y = new int[4];
Calibration cal = imp.getCalibration();
double cx = cal.pixelWidth; //corrects preview for x calibration
double cy = cal.pixelHeight; //corrects preview for y calibration
x[0] = (int)gx1;
y[0] = (int)gy1;
x[1] = (int)gx2;
y[1] = (int)gy2;
double dx = gx2 - gx1;
double dy = gy2 - gy1;
double nrm = Math.sqrt(dx*dx + dy*dy)/outSpacing;
double xInc = -(dy/(cx*nrm)); //cx scales the x increment
double yInc = (dx/(cy*nrm)); //cy scales the y increment
x[2] = x[1] + (int)(xInc*count);
y[2] = y[1] + (int)(yInc*count);
x[3] = x[0] + (int)(xInc*count);
y[3] = y[0] + (int)(yInc*count);
imp.setRoi(new PolygonRoi(x, y, 4, PolygonRoi.FREEROI));
}
int getOutputStackSize(double inSpacing, double outSpacing, int count) {
Roi roi = imp.getRoi();
int width = imp.getWidth();
int height = imp.getHeight();
if (roi!=null) {
Rectangle r = roi.getBounds();
width = r.width;
width = r.height;
}
int type = roi!=null?roi.getType():0;
int stackSize = imp.getStackSize();
double size = 0.0;
if (type==Roi.RECTANGLE) {
size = width*height*stackSize;
if (outSpacing>0&&!nointerpolate) size *= inSpacing/outSpacing;
} else
size = gLength*count*stackSize;
int bits = imp.getBitDepth();
switch (bits) {
case 16: size*=2; break;
case 24: case 32: size*=4; break;
}
return (int)Math.round(size/1048576.0);
}
int getAvailableMemory() {
long max = IJ.maxMemory();
if (max==0) return -1;
long inUse = IJ.currentMemory();
long available = max - inUse;
return (int)((available+524288L)/1048576L);
}
}
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