package ij.plugin;
import ij.*;
import ij.gui.*;
import ij.process.*;
import ij.measure.*;
import ij.plugin.frame.*;
import ij.macro.Interpreter;
import ij.plugin.filter.*;
import ij.util.Tools;
import java.awt.*;
import java.awt.event.KeyEvent;
import java.util.Vector;
/** This plugin implements the commands in the Edit/Selection submenu. */
public class Selection implements PlugIn, Measurements {
private ImagePlus imp;
private float[] kernel = {1f, 1f, 1f, 1f, 1f};
private float[] kernel3 = {1f, 1f, 1f};
private static int bandSize = 15; // pixels
private static Color linec, fillc;
private static int lineWidth = 1;
private static boolean smooth;
private static boolean adjust;
public void run(String arg) {
imp = WindowManager.getCurrentImage();
if (arg.equals("add"))
{addToRoiManager(imp); return;}
if (imp==null)
{IJ.noImage(); return;}
if (arg.equals("all"))
imp.setRoi(0,0,imp.getWidth(),imp.getHeight());
else if (arg.equals("none"))
imp.deleteRoi();
else if (arg.equals("restore"))
imp.restoreRoi();
else if (arg.equals("spline"))
fitSpline();
else if (arg.equals("interpolate"))
interpolate();
else if (arg.equals("circle"))
fitCircle(imp);
else if (arg.equals("ellipse"))
createEllipse(imp);
else if (arg.equals("hull"))
convexHull(imp);
else if (arg.equals("mask"))
createMask(imp);
else if (arg.equals("from"))
createSelectionFromMask(imp);
else if (arg.equals("inverse"))
invert(imp);
else if (arg.equals("toarea"))
lineToArea(imp);
else if (arg.equals("toline"))
areaToLine(imp);
else if (arg.equals("properties"))
{setProperties("Properties ", imp.getRoi()); imp.draw();}
else if (arg.equals("band"))
makeBand(imp);
else if (arg.equals("tobox"))
toBoundingBox(imp);
else if (arg.equals("rotate"))
rotate(imp);
else if (arg.equals("enlarge"))
enlarge(imp);
}
private void rotate(ImagePlus imp) {
Roi roi = imp.getRoi();
if (IJ.macroRunning()) {
String options = Macro.getOptions();
if (options!=null && (options.indexOf("grid=")!=-1||options.indexOf("interpolat")!=-1)) {
IJ.run("Rotate... ", options); // run Image>Transform>Rotate
return;
}
}
(new RoiRotator()).run("");
}
private void enlarge(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi!=null) {
Undo.setup(Undo.ROI, imp);
roi = (Roi)roi.clone();
(new RoiEnlarger()).run("");
} else
noRoi("Enlarge");
}
/*
if selection is closed shape, create a circle with the same area and centroid, otherwise use
the Pratt method to fit a circle to the points that define the line or multi-point selection.
Reference: Pratt V., Direct least-squares fitting of algebraic surfaces", Computer Graphics, Vol. 21, pages 145-152 (1987).
Original code: Nikolai Chernov's MATLAB script for Newton-based Pratt fit.
(http://www.math.uab.edu/~chernov/cl/MATLABcircle.html)
Java version: https://github.com/mdoube/BoneJ/blob/master/src/org/doube/geometry/FitCircle.java
Authors: Nikolai Chernov, Michael Doube, Ved Sharma
*/
void fitCircle(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null) {
noRoi("Fit Circle");
return;
}
if (roi.isArea()) { //create circle with the same area and centroid
Undo.setup(Undo.ROI, imp);
ImageProcessor ip = imp.getProcessor();
ip.setRoi(roi);
ImageStatistics stats = ImageStatistics.getStatistics(ip, Measurements.AREA+Measurements.CENTROID, null);
double r = Math.sqrt(stats.pixelCount/Math.PI);
imp.deleteRoi();
int d = (int)Math.round(2.0*r);
Roi roi2 = new OvalRoi((int)Math.round(stats.xCentroid-r), (int)Math.round(stats.yCentroid-r), d, d);
transferProperties(roi, roi2);
imp.setRoi(roi2);
return;
}
Polygon poly = roi.getPolygon();
int n=poly.npoints;
int[] x = poly.xpoints;
int[] y = poly.ypoints;
if (n<3) {
IJ.error("Fit Circle", "At least 3 points are required to fit a circle.");
return;
}
// calculate point centroid
double sumx = 0, sumy = 0;
for (int i=0; iMath.abs(yold)) {
if (IJ.debugMode) IJ.log("Fit Circle: wrong direction: |ynew| > |yold|");
xnew = 0;
break;
}
double Dy = A1 + xnew*(A22 + 16*xnew*xnew);
double xold = xnew;
xnew = xold - ynew/Dy;
if (Math.abs((xnew-xold)/xnew) < epsilon)
break;
if (iter >= IterMax) {
if (IJ.debugMode) IJ.log("Fit Circle: will not converge");
xnew = 0;
}
if (xnew<0) {
if (IJ.debugMode) IJ.log("Fit Circle: negative root: x = "+xnew);
xnew = 0;
}
}
if (IJ.debugMode) IJ.log("Fit Circle: n="+n+", xnew="+IJ.d2s(xnew,2)+", iterations="+iterations);
// calculate the circle parameters
double DET = xnew*xnew - xnew*Mz + Cov_xy;
double CenterX = (Mxz*(Myy-xnew)-Myz*Mxy)/(2*DET);
double CenterY = (Myz*(Mxx-xnew)-Mxz*Mxy)/(2*DET);
double radius = Math.sqrt(CenterX*CenterX + CenterY*CenterY + Mz + 2*xnew);
if (Double.isNaN(radius)) {
IJ.error("Fit Circle", "Points are collinear.");
return;
}
CenterX = CenterX + meanx;
CenterY = CenterY + meany;
Undo.setup(Undo.ROI, imp);
imp.deleteRoi();
IJ.makeOval((int)Math.round(CenterX-radius), (int)Math.round(CenterY-radius), (int)Math.round(2*radius), (int)Math.round(2*radius));
}
void fitSpline() {
Roi roi = imp.getRoi();
if (roi==null)
{noRoi("Spline"); return;}
int type = roi.getType();
boolean segmentedSelection = type==Roi.POLYGON||type==Roi.POLYLINE;
if (!(segmentedSelection||type==Roi.FREEROI||type==Roi.TRACED_ROI||type==Roi.FREELINE))
{IJ.error("Spline Fit", "Polygon or polyline selection required"); return;}
if (roi instanceof EllipseRoi)
return;
PolygonRoi p = (PolygonRoi)roi;
Undo.setup(Undo.ROI, imp);
if (!segmentedSelection && p.getNCoordinates()>3) {
if (p.subPixelResolution())
p = trimFloatPolygon(p, p.getUncalibratedLength());
else
p = trimPolygon(p, p.getUncalibratedLength());
}
String options = Macro.getOptions();
if (options!=null && options.indexOf("straighten")!=-1)
p.fitSplineForStraightening();
else if (options!=null && options.indexOf("remove")!=-1)
p.removeSplineFit();
else
p.fitSpline();
imp.draw();
LineWidthAdjuster.update();
}
void interpolate() {
Roi roi = imp.getRoi();
if (roi==null)
{noRoi("Interpolate"); return;}
if (roi.getType()==Roi.POINT)
return;
if (IJ.isMacro()&&Macro.getOptions()==null)
Macro.setOptions("interval=1");
GenericDialog gd = new GenericDialog("Interpolate");
gd.addNumericField("Interval:", 1.0, 1, 4, "pixel");
gd.addCheckbox("Smooth", IJ.isMacro()?false:smooth);
gd.addCheckbox("Adjust interval to match", IJ.isMacro()?false:adjust);
gd.showDialog();
if (gd.wasCanceled())
return;
double interval = gd.getNextNumber();
smooth = gd.getNextBoolean();
Undo.setup(Undo.ROI, imp);
adjust = gd.getNextBoolean();
int sign = adjust ? -1 : 1;
FloatPolygon poly = roi.getInterpolatedPolygon(sign*interval, smooth);
int t = roi.getType();
int type = roi.isLine()?Roi.FREELINE:Roi.FREEROI;
if (t==Roi.POLYGON && interval>1.0)
type = Roi.POLYGON;
if ((t==Roi.RECTANGLE||t==Roi.OVAL||t==Roi.FREEROI) && interval>=8.0)
type = Roi.POLYGON;
if ((t==Roi.LINE||t==Roi.FREELINE) && interval>=8.0)
type = Roi.POLYLINE;
if (t==Roi.POLYLINE && interval>=8.0)
type = Roi.POLYLINE;
ImageCanvas ic = imp.getCanvas();
if (poly.npoints<=150 && ic!=null && ic.getMagnification()>=12.0)
type = roi.isLine()?Roi.POLYLINE:Roi.POLYGON;
Roi p = new PolygonRoi(poly,type);
if (roi.getStroke()!=null)
p.setStrokeWidth(roi.getStrokeWidth());
p.setStrokeColor(roi.getStrokeColor());
p.setName(roi.getName());
transferProperties(roi, p);
imp.setRoi(p);
}
private static void transferProperties(Roi roi1, Roi roi2) {
if (roi1==null || roi2==null)
return;
roi2.setStrokeColor(roi1.getStrokeColor());
if (roi1.getStroke()!=null)
roi2.setStroke(roi1.getStroke());
roi2.setDrawOffset(roi1.getDrawOffset());
}
PolygonRoi trimPolygon(PolygonRoi roi, double length) {
int[] x = roi.getXCoordinates();
int[] y = roi.getYCoordinates();
int n = roi.getNCoordinates();
x = smooth(x, n);
y = smooth(y, n);
float[] curvature = getCurvature(x, y, n);
Rectangle r = roi.getBounds();
double threshold = rodbard(length);
//IJ.log("trim: "+length+" "+threshold);
double distance = Math.sqrt((x[1]-x[0])*(x[1]-x[0])+(y[1]-y[0])*(y[1]-y[0]));
x[0] += r.x; y[0]+=r.y;
int i2 = 1;
int x1,y1,x2=0,y2=0;
for (int i=1; i=threshold) {
x[i2] = x2 + r.x;
y[i2] = y2 + r.y;
i2++;
distance = 0.0;
}
}
int type = roi.getType()==Roi.FREELINE?Roi.POLYLINE:Roi.POLYGON;
if (type==Roi.POLYLINE && distance>0.0) {
x[i2] = x2 + r.x;
y[i2] = y2 + r.y;
i2++;
}
PolygonRoi p = new PolygonRoi(x, y, i2, type);
if (roi.getStroke()!=null)
p.setStrokeWidth(roi.getStrokeWidth());
p.setStrokeColor(roi.getStrokeColor());
p.setName(roi.getName());
imp.setRoi(p);
return p;
}
double rodbard(double x) {
// y = c*((a-x/(x-d))^(1/b)
// a=3.9, b=.88, c=712, d=44
double ex;
if (x == 0.0)
ex = 5.0;
else
ex = Math.exp(Math.log(x/700.0)*0.88);
double y = 3.9-44.0;
y = y/(1.0+ex);
return y+44.0;
}
int[] smooth(int[] a, int n) {
FloatProcessor fp = new FloatProcessor(n, 1);
for (int i=0; i=threshold) {
x[i2] = (float)x2;
y[i2] = (float)y2;
i2++;
distance = 0.0;
}
}
int type = roi.getType()==Roi.FREELINE?Roi.POLYLINE:Roi.POLYGON;
if (type==Roi.POLYLINE && distance>0.0) {
x[i2] = (float)x2;
y[i2] = (float)y2;
i2++;
}
PolygonRoi p = new PolygonRoi(x, y, i2, type);
if (roi.getStroke()!=null)
p.setStrokeWidth(roi.getStrokeWidth());
p.setStrokeColor(roi.getStrokeColor());
p.setDrawOffset(roi.getDrawOffset());
p.setName(roi.getName());
imp.setRoi(p);
return p;
}
float[] smooth(float[] a, int n) {
FloatProcessor fp = new FloatProcessor(n, 1);
for (int i=0; iAdjust>Threshold tool. The current\n"+
"image is not a mask and has not been thresholded.");
return;
}
int threshold = ip.isInvertedLut()?255:0;
ip.setThreshold(threshold, threshold, ImageProcessor.NO_LUT_UPDATE);
IJ.runPlugIn("ij.plugin.filter.ThresholdToSelection", "");
}
void invert(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null || !roi.isArea())
{IJ.error("Inverse", "Area selection required"); return;}
ShapeRoi s1, s2;
if (roi instanceof ShapeRoi)
s1 = (ShapeRoi)roi;
else
s1 = new ShapeRoi(roi);
s2 = new ShapeRoi(new Roi(0,0, imp.getWidth(), imp.getHeight()));
Undo.setup(Undo.ROI, imp);
imp.setRoi(s1.xor(s2));
}
private void lineToArea(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null || !roi.isLine())
{IJ.error("Line to Area", "Line selection required"); return;}
Undo.setup(Undo.ROI, imp);
Roi roi2 = lineToArea(roi);
imp.setRoi(roi2);
Roi.previousRoi = (Roi)roi.clone();
}
/** Converts a line selection into an area selection. */
public static Roi lineToArea(Roi roi) {
Roi roi2 = null;
if (roi.getType()==Roi.LINE) {
double width = roi.getStrokeWidth();
if (width<=1.0)
roi.setStrokeWidth(1.0000001);
FloatPolygon p = roi.getFloatPolygon();
roi.setStrokeWidth(width);
roi2 = new PolygonRoi(p, Roi.POLYGON);
roi2.setDrawOffset(roi.getDrawOffset());
} else {
roi = (Roi)roi.clone();
int lwidth = (int)roi.getStrokeWidth();
if (lwidth<1)
lwidth = 1;
Rectangle bounds = roi.getBounds();
int width = bounds.width + lwidth*2;
int height = bounds.height + lwidth*2;
ImageProcessor ip2 = new ByteProcessor(width, height);
roi.setLocation(lwidth, lwidth);
ip2.setColor(255);
roi.drawPixels(ip2);
ip2.setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
ThresholdToSelection tts = new ThresholdToSelection();
roi2 = tts.convert(ip2);
if (roi2==null)
return roi;
if (bounds.x==0&&bounds.y==0)
roi2.setLocation(0, 0);
else
roi2.setLocation(bounds.x-lwidth/2, bounds.y-lwidth/2);
}
transferProperties(roi, roi2);
roi2.setStrokeWidth(0);
Color c = roi2.getStrokeColor();
if (c!=null) // remove any transparency
roi2.setStrokeColor(new Color(c.getRed(),c.getGreen(),c.getBlue()));
return roi2;
}
void areaToLine(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null || !roi.isArea()) {
IJ.error("Area to Line", "Area selection required");
return;
}
Undo.setup(Undo.ROI, imp);
Polygon p = roi.getPolygon();
if (p==null) return;
int type1 = roi.getType();
if (type1==Roi.COMPOSITE) {
IJ.error("Area to Line", "Composite selections cannot be converted to lines.");
return;
}
int type2 = Roi.POLYLINE;
if (type1==Roi.OVAL||type1==Roi.FREEROI||type1==Roi.TRACED_ROI
||((roi instanceof PolygonRoi)&&((PolygonRoi)roi).isSplineFit()))
type2 = Roi.FREELINE;
Roi roi2 = new PolygonRoi(p.xpoints, p.ypoints, p.npoints, type2);
transferProperties(roi, roi2);
imp.setRoi(roi2);
}
void toBoundingBox(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null) {
noRoi("To Bounding Box");
return;
}
Undo.setup(Undo.ROI, imp);
Rectangle r = roi.getBounds();
imp.deleteRoi();
Roi roi2 = new Roi(r.x, r.y, r.width, r.height);
transferProperties(roi, roi2);
imp.setRoi(roi2);
}
void addToRoiManager(ImagePlus imp) {
if (IJ.macroRunning() && Interpreter.isBatchModeRoiManager())
IJ.error("run(\"Add to Manager\") may not work in batch mode macros");
Frame frame = WindowManager.getFrame("ROI Manager");
if (frame==null)
IJ.run("ROI Manager...");
if (imp==null) return;
Roi roi = imp.getRoi();
if (roi==null) return;
frame = WindowManager.getFrame("ROI Manager");
if (frame==null || !(frame instanceof RoiManager))
IJ.error("ROI Manager not found");
RoiManager rm = (RoiManager)frame;
boolean altDown= IJ.altKeyDown();
IJ.setKeyUp(IJ.ALL_KEYS);
if (altDown && !IJ.macroRunning())
IJ.setKeyDown(KeyEvent.VK_SHIFT);
if (roi.getState()==Roi.CONSTRUCTING) { //wait (up to 2 sec.) until ROI finished
long start = System.currentTimeMillis();
while (true) {
IJ.wait(10);
if (roi.getState()!=Roi.CONSTRUCTING)
break;
if ((System.currentTimeMillis()-start)>2000) {
IJ.beep();
IJ.error("Add to Manager", "Selection is not complete");
return;
}
}
}
rm.allowRecording(true);
rm.runCommand("add");
rm.allowRecording(false);
IJ.setKeyUp(IJ.ALL_KEYS);
}
boolean setProperties(String title, Roi roi) {
if ((roi instanceof PointRoi) && Toolbar.getMultiPointMode() && IJ.altKeyDown()) {
((PointRoi)roi).displayCounts();
return true;
}
Frame f = WindowManager.getFrontWindow();
if (f!=null && f.getTitle().indexOf("3D Viewer")!=-1)
return false;
if (roi==null) {
IJ.error("This command requires a selection.");
return false;
}
RoiProperties rp = new RoiProperties(title, roi);
boolean ok = rp.showDialog();
if (IJ.debugMode)
IJ.log(roi.getDebugInfo());
return ok;
}
private void makeBand(ImagePlus imp) {
Roi roi = imp.getRoi();
if (roi==null) {
noRoi("Make Band");
return;
}
Roi roiOrig = roi;
if (!roi.isArea()) {
IJ.error("Make Band", "Area selection required");
return;
}
Calibration cal = imp.getCalibration();
double pixels = bandSize;
double size = pixels*cal.pixelWidth;
int decimalPlaces = 0;
if ((int)size!=size)
decimalPlaces = 2;
GenericDialog gd = new GenericDialog("Make Band");
gd.addNumericField("Band Size:", size, decimalPlaces, 4, cal.getUnits());
gd.showDialog();
if (gd.wasCanceled())
return;
size = gd.getNextNumber();
if (Double.isNaN(size)) {
IJ.error("Make Band", "invalid number");
return;
}
int n = (int)Math.round(size/cal.pixelWidth);
if (n >255) {
IJ.error("Make Band", "Cannot make bands wider that 255 pixels");
return;
}
int width = imp.getWidth();
int height = imp.getHeight();
Rectangle r = roi.getBounds();
ImageProcessor ip = roi.getMask();
if (ip==null) {
ip = new ByteProcessor(r.width, r.height);
ip.invert();
}
ImageProcessor mask = new ByteProcessor(width, height);
mask.insert(ip, r.x, r.y);
ImagePlus edm = new ImagePlus("mask", mask);
boolean saveBlackBackground = Prefs.blackBackground;
Prefs.blackBackground = false;
int saveType = EDM.getOutputType();
EDM.setOutputType(EDM.BYTE_OVERWRITE);
IJ.run(edm, "Distance Map", "");
EDM.setOutputType(saveType);
Prefs.blackBackground = saveBlackBackground;
ip = edm.getProcessor();
ip.setThreshold(0, n, ImageProcessor.NO_LUT_UPDATE);
int xx=-1, yy=-1;
for (int x=r.x; x=0||yy>=0)
break;
}
int count = IJ.doWand(edm, xx, yy, 0, null);
if (count<=0) {
IJ.error("Make Band", "Unable to make band");
return;
}
ShapeRoi roi2 = new ShapeRoi(edm.getRoi());
if (!(roi instanceof ShapeRoi))
roi = new ShapeRoi(roi);
ShapeRoi roi1 = (ShapeRoi)roi;
roi2 = roi2.not(roi1);
Undo.setup(Undo.ROI, imp);
transferProperties(roiOrig, roi2);
imp.setRoi(roi2);
bandSize = n;
}
void noRoi(String command) {
IJ.error(command, "This command requires a selection");
}
}
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