package ij.plugin;
import ij.*;
import ij.gui.*;
import ij.measure.*;
import ij.process.*;
import java.awt.*;
import java.awt.image.*;
import java.awt.event.*;
import java.awt.geom.*;
import java.util.*;
/**
* This plugin projects dynamically orthogonal XZ and YZ views of a stack.
* The output images are calibrated, which allows measurements to be performed more easily.
*
* Many thanks to Jerome Mutterer for the code contributions and testing.
* Thanks to Wayne Rasband for the code that properly handles the image magnification.
*
* @author Dimiter Prodanov
*/
public class Orthogonal_Views implements PlugIn, MouseListener, MouseMotionListener, KeyListener, ActionListener,
ImageListener, WindowListener, AdjustmentListener, MouseWheelListener, FocusListener, CommandListener, Runnable {
private ImageWindow win;
private ImagePlus imp;
private boolean rgb;
private ImageStack imageStack;
private boolean hyperstack;
private int currentChannel, currentFrame, currentMode;
private ImageCanvas canvas;
private static final int H_ROI=0, H_ZOOM=1;
private static boolean sticky=true;
private static int xzID, yzID;
private static Orthogonal_Views instance;
private ImagePlus xz_image, yz_image;
/** ImageProcessors for the xz and yz images */
private ImageProcessor fp1, fp2;
private double ax, ay, az;
private boolean rotateYZ = Prefs.rotateYZ;
private boolean flipXZ = Prefs.flipXZ;
private int xyX, xyY;
private Calibration cal=null, cal_xz=new Calibration(), cal_yz=new Calibration();
private double magnification=1.0;
private Color color = Roi.getColor();
private double min, max;
private Dimension screen = IJ.getScreenSize();
private boolean syncZoom = true;
private Point crossLoc;
private boolean firstTime = true;
private static int previousID, previousX, previousY;
private Rectangle startingSrcRect;
private boolean done;
private boolean initialized;
private boolean sliceSet;
private Thread thread;
public void run(String arg) {
imp = IJ.getImage();
boolean isStack = imp.getStackSize()>1;
hyperstack = imp.isHyperStack();
if ((hyperstack||imp.isComposite()) && imp.getNSlices()<=1)
isStack = false;
if (instance!=null) {
if (imp==instance.imp) {
instance.dispose();
return;
} else if (isStack) {
instance.dispose();
if (IJ.isMacro()) IJ.wait(1000);
} else {
ImageWindow win = instance.imp!=null?instance.imp.getWindow():null;
if (win!=null) win.toFront();
return;
}
}
if (!isStack) {
IJ.error("Othogonal Views", "This command requires a stack, or a hypertack with Z>1.");
return;
}
yz_image = WindowManager.getImage(yzID);
rgb = imp.getBitDepth()==24 || hyperstack;
int yzBitDepth = hyperstack?24:imp.getBitDepth();
if (yz_image==null || yz_image.getHeight()!=imp.getHeight() || yz_image.getBitDepth()!=yzBitDepth)
yz_image = new ImagePlus();
xz_image = WindowManager.getImage(xzID);
if (xz_image==null || xz_image.getWidth()!=imp.getWidth() || xz_image.getBitDepth()!=yzBitDepth)
xz_image = new ImagePlus();
instance = this;
int mode = imp.getCompositeMode();
ImageProcessor ip = mode==IJ.COMPOSITE?new ColorProcessor(imp.getImage()):imp.getProcessor();
min = ip.getMin();
max = ip.getMax();
cal=this.imp.getCalibration();
double calx=cal.pixelWidth;
double caly=cal.pixelHeight;
double calz=cal.pixelDepth;
ax = 1.0;
ay = caly/calx;
az = calz/calx;
if (az>100) {
IJ.error("Z spacing ("+(int)az+") is too large.");
return;
}
win = imp.getWindow();
canvas = win.getCanvas();
addListeners(canvas);
magnification= canvas.getMagnification();
imp.deleteRoi();
Rectangle r = canvas.getSrcRect();
if (imp.getID()==previousID)
crossLoc = new Point(previousX, previousY);
else
crossLoc = new Point(r.x+r.width/2, r.y+r.height/2);
imageStack = getStack();
calibrate();
if (createProcessors(imageStack)) {
if (ip.isColorLut() || ip.isInvertedLut()) {
ColorModel cm = ip.getColorModel();
fp1.setColorModel(cm);
fp2.setColorModel(cm);
}
thread = new Thread(this, "Orthogonal Views");
thread.start();
IJ.wait(100);
update();
} else
dispose();
}
private ImageStack getStack() {
if (imp.isHyperStack()) {
int slices = imp.getNSlices();
int c=imp.getChannel();
int z=imp.getSlice();
int t=imp.getFrame();
int mode = imp.getCompositeMode();
rgb = mode==IJ.COMPOSITE;
ColorModel cm = rgb?null:imp.getProcessor().getColorModel();
//IJ.log("getStack; "+c+" "+currentChannel+" "+fp1);
if (cm!=null && fp1!=null && fp1.getBitDepth()!=24) {
fp1.setColorModel(cm);
fp2.setColorModel(cm);
}
ImageStack stack = imp.getStack();
ImageStack stack2 = new ImageStack(imp.getWidth(), imp.getHeight());
for (int i=1; i<=slices; i++) {
if (rgb) {
imp.setPositionWithoutUpdate(c, i, t);
stack2.addSlice(null, new ColorProcessor(imp.getImage()));
} else {
int index = imp.getStackIndex(c, i, t);
stack2.addSlice(null, stack.getProcessor(index));
}
}
if (rgb)
imp.setPosition(c, z, t);
currentChannel = c;
currentFrame = t;
currentMode = mode;
return stack2;
} else
return imp.getStack();
}
private void addListeners(ImageCanvas canvas) {
canvas.addMouseListener(this);
canvas.addMouseMotionListener(this);
canvas.addKeyListener(this);
win.addWindowListener (this);
win.addMouseWheelListener(this);
win.addFocusListener(this);
ImagePlus.addImageListener(this);
Executer.addCommandListener(this);
}
private void calibrate() {
String unit=cal.getUnit();
double o_depth=cal.pixelDepth;
double o_height=cal.pixelHeight;
double o_width=cal.pixelWidth;
cal_yz.setUnit(unit);
if (rotateYZ) {
cal_yz.pixelHeight=o_depth/az;
cal_yz.pixelWidth=o_height;
} else {
cal_yz.pixelWidth=o_depth/az;
cal_yz.pixelHeight=o_height;
}
yz_image.setCalibration(cal_yz);
yz_image.setIJMenuBar(false);
cal_xz.setUnit(unit);
cal_xz.pixelWidth=o_width;
cal_xz.pixelHeight=o_depth/az;
xz_image.setCalibration(cal_xz);
xz_image.setIJMenuBar(false);
}
private void updateMagnification(int x, int y) {
double magnification= win.getCanvas().getMagnification();
int z = imp.getSlice()-1;
ImageWindow xz_win = xz_image.getWindow();
if (xz_win==null) return;
ImageCanvas xz_ic = xz_win.getCanvas();
double xz_mag = xz_ic.getMagnification();
double arat = az/ax;
int zcoord=(int)(arat*z);
if (flipXZ) zcoord=(int)(arat*(imp.getNSlices()-z));
while (xz_magmagnification) {
xz_ic.zoomOut(xz_ic.screenX(x), xz_ic.screenY(zcoord));
xz_mag = xz_ic.getMagnification();
}
ImageWindow yz_win = yz_image.getWindow();
if (yz_win==null) return;
ImageCanvas yz_ic = yz_win.getCanvas();
double yz_mag = yz_ic.getMagnification();
zcoord = (int)(arat*z);
while (yz_magmagnification) {
yz_ic.zoomOut(yz_ic.screenX(zcoord), yz_ic.screenY(y));
yz_mag = yz_ic.getMagnification();
}
}
void updateViews(Point p, ImageStack is) {
if (fp1==null) return;
updateXZView(p,is);
double arat=az/ax;
int width2 = fp1.getWidth();
int height2 = (int)Math.round(fp1.getHeight()*az);
if (height2<1) height2=1;
if (width2!=fp1.getWidth()||height2!=fp1.getHeight()) {
fp1.setInterpolate(true);
ImageProcessor sfp1=fp1.resize(width2, height2);
if (!rgb) sfp1.setMinAndMax(min, max);
xz_image.setProcessor("XZ "+p.y, sfp1);
} else {
if (!rgb) fp1.setMinAndMax(min, max);
xz_image.setProcessor("XZ "+p.y, fp1);
}
if (rotateYZ)
updateYZView(p, is);
else
updateZYView(p, is);
width2 = (int)Math.round(fp2.getWidth()*az);
if (width2<1) width2=1;
height2 = fp2.getHeight();
String title = "YZ ";
if (rotateYZ) {
width2 = fp2.getWidth();
height2 = (int)Math.round(fp2.getHeight()*az);
if (height2<1) height2=1;
title = "ZY ";
}
if (width2!=fp2.getWidth()||height2!=fp2.getHeight()) {
fp2.setInterpolate(true);
ImageProcessor sfp2=fp2.resize(width2, height2);
if (!rgb) sfp2.setMinAndMax(min, max);
yz_image.setProcessor(title+p.x, sfp2);
} else {
if (!rgb) fp2.setMinAndMax(min, max);
yz_image.setProcessor(title+p.x, fp2);
}
calibrate();
if (yz_image.getWindow()==null) {
yz_image.show();
ImageCanvas ic = yz_image.getCanvas();
ic.addKeyListener(this);
ic.addMouseListener(this);
//ic.addMouseMotionListener(this);
ic.setCustomRoi(true);
//yz_image.getWindow().addMouseWheelListener(this);
yzID = yz_image.getID();
} else {
ImageCanvas ic = yz_image.getWindow().getCanvas();
ic.addMouseListener(this);
//ic.addMouseMotionListener(this);
ic.setCustomRoi(true);
}
if (xz_image.getWindow()==null) {
xz_image.show();
ImageCanvas ic = xz_image.getCanvas();
ic.addKeyListener(this);
ic.addMouseListener(this);
//ic.addMouseMotionListener(this);
ic.setCustomRoi(true);
//xz_image.getWindow().addMouseWheelListener(this);
xzID = xz_image.getID();
} else {
ImageCanvas ic = xz_image.getWindow().getCanvas();
ic.addMouseListener(this);
//ic.addMouseMotionListener(this);
ic.setCustomRoi(true);
}
}
void arrangeWindows(boolean sticky) {
ImageWindow xyWin = imp.getWindow();
if (xyWin==null) return;
Point loc = xyWin.getLocation();
if ((xyX!=loc.x)||(xyY!=loc.y)) {
xyX = loc.x;
xyY = loc.y;
ImageWindow yzWin =null;
long start = System.currentTimeMillis();
while (yzWin==null && (System.currentTimeMillis()-start)<=2500L) {
yzWin = yz_image.getWindow();
if (yzWin==null) IJ.wait(50);
}
if (yzWin!=null)
yzWin.setLocation(xyX+xyWin.getWidth(), xyY);
ImageWindow xzWin =null;
start = System.currentTimeMillis();
while (xzWin==null && (System.currentTimeMillis()-start)<=2500L) {
xzWin = xz_image.getWindow();
if (xzWin==null) IJ.wait(50);
}
if (xzWin!=null)
xzWin.setLocation(xyX,xyY+xyWin.getHeight());
if (firstTime) {
imp.getWindow().toFront();
if (!sliceSet) {
if (hyperstack)
imp.setPosition(imp.getChannel(), imp.getNSlices()/2, imp.getFrame());
else
imp.setSlice(imp.getNSlices()/2);
}
firstTime = false;
}
}
}
/**
* @param is - used to get the dimensions of the new ImageProcessors
* @return
*/
boolean createProcessors(ImageStack is) {
ImageProcessor ip=is.getProcessor(1);
int width= is.getWidth();
int height=is.getHeight();
int ds=is.getSize();
double arat=1.0;//az/ax;
double brat=1.0;//az/ay;
int za=(int)(ds*arat);
int zb=(int)(ds*brat);
if (ip instanceof FloatProcessor) {
fp1=new FloatProcessor(width,za);
if (rotateYZ)
fp2=new FloatProcessor(height,zb);
else
fp2=new FloatProcessor(zb,height);
return true;
}
if (ip instanceof ByteProcessor) {
fp1=new ByteProcessor(width,za);
if (rotateYZ)
fp2=new ByteProcessor(height,zb);
else
fp2=new ByteProcessor(zb,height);
return true;
}
if (ip instanceof ShortProcessor) {
fp1=new ShortProcessor(width,za);
if (rotateYZ)
fp2=new ShortProcessor(height,zb);
else
fp2=new ShortProcessor(zb,height);
return true;
}
if (ip instanceof ColorProcessor) {
fp1=new ColorProcessor(width,za);
if (rotateYZ)
fp2=new ColorProcessor(height,zb);
else
fp2=new ColorProcessor(zb,height);
return true;
}
return false;
}
void updateXZView(Point p, ImageStack is) {
int width= is.getWidth();
int size=is.getSize();
ImageProcessor ip=is.getProcessor(1);
int y=p.y;
// XZ
if (ip instanceof ShortProcessor) {
short[] newpix=new short[width*size];
for (int i=0; iyz_image.getWidth()-yzSrcRect.width)
yzSrcRect.y = yz_image.getWidth()-yzSrcRect.width;
} else {
yzSrcRect.y += dy;
if (yzSrcRect.y<0)
yzSrcRect.y = 0;
if (yzSrcRect.y>yz_image.getHeight()-yzSrcRect.height)
yzSrcRect.y = yz_image.getHeight()-yzSrcRect.height;
}
yzic.repaint();
int dx = srcRect.x - startingSrcRect.x;
ImageCanvas xzic = xz_image.getCanvas();
Rectangle xzSrcRect =xzic.getSrcRect();
xzSrcRect.x += dx;
if (xzSrcRect.x<0)
xzSrcRect.x = 0;
if (xzSrcRect.x>xz_image.getWidth()-xzSrcRect.width)
xzSrcRect.x = xz_image.getWidth()-xzSrcRect.width;
xzic.repaint();
}
}
/** Refresh the output windows. */
synchronized void update() {
notify();
}
private void exec() {
if (canvas==null)
return;
int width=imp.getWidth();
int height=imp.getHeight();
if (hyperstack) {
int mode = IJ.COMPOSITE;
if (imp.isComposite()) {
mode = ((CompositeImage)imp).getMode();
if (mode!=currentMode)
imageStack = null;
}
if (imageStack!=null) {
int c = imp.getChannel();
int t = imp.getFrame();
if ((mode!=IJ.COMPOSITE&&c!=currentChannel) || t!=currentFrame)
imageStack = null;
}
}
ImageStack is = imageStack;
if (is==null)
is = imageStack = getStack();
double arat=az/ax;
double brat=az/ay;
Point p=crossLoc;
if (p.y>=height) p.y=height-1;
if (p.x>=width) p.x=width-1;
if (p.x<0) p.x=0;
if (p.y<0) p.y=0;
updateViews(p, is);
GeneralPath path = new GeneralPath();
drawCross(imp, p, path);
if (!done)
imp.setOverlay(path, color, new BasicStroke(1));
canvas.setCustomRoi(true);
updateCrosses(p.x, p.y, arat, brat);
if (syncZoom) updateMagnification(p.x, p.y);
arrangeWindows(sticky);
initialized = true;
}
private void updateCrosses(int x, int y, double arat, double brat) {
Point p;
int z=imp.getNSlices();
int zlice=imp.getSlice()-1;
int zcoord=(int)Math.round(arat*zlice);
if (flipXZ) zcoord = (int)Math.round(arat*(z-zlice));
ImageCanvas xzCanvas = xz_image.getCanvas();
p=new Point (x, zcoord);
GeneralPath path = new GeneralPath();
drawCross(xz_image, p, path);
if (!done)
xz_image.setOverlay(path, color, new BasicStroke(1));
if (rotateYZ) {
if (flipXZ)
zcoord=(int)Math.round(brat*(z-zlice));
else
zcoord=(int)Math.round(brat*(zlice));
p=new Point (y, zcoord);
} else {
zcoord=(int)Math.round(arat*zlice);
p=new Point (zcoord, y);
}
path = new GeneralPath();
drawCross(yz_image, p, path);
if (!done)
yz_image.setOverlay(path, color, new BasicStroke(1));
IJ.showStatus(imp.getLocationAsString(crossLoc.x, crossLoc.y));
}
public void mouseMoved(MouseEvent e) {
}
public void keyPressed(KeyEvent e) {
int key = e.getKeyCode();
if (key==KeyEvent.VK_ESCAPE) {
IJ.beep();
dispose();
} else if (IJ.shiftKeyDown()) {
int width=imp.getWidth(), height=imp.getHeight();
switch (key) {
case KeyEvent.VK_LEFT: crossLoc.x--; if (crossLoc.x<0) crossLoc.x=0; break;
case KeyEvent.VK_RIGHT: crossLoc.x++; if (crossLoc.x>=width) crossLoc.x=width-1; break;
case KeyEvent.VK_UP: crossLoc.y--; if (crossLoc.y<0) crossLoc.y=0; break;
case KeyEvent.VK_DOWN: crossLoc.y++; if (crossLoc.y>=height) crossLoc.y=height-1; break;
default: return;
}
update();
}
}
public void keyReleased(KeyEvent e) {
}
public void keyTyped(KeyEvent e) {
}
public void actionPerformed(ActionEvent ev) {
}
public void imageClosed(ImagePlus imp) {
if (!done)
dispose();
}
public void imageOpened(ImagePlus imp) {
}
public void imageUpdated(ImagePlus imp) {
if (imp==this.imp) {
ImageProcessor ip = imp.getProcessor();
min = ip.getMin();
max = ip.getMax();
update();
}
}
public String commandExecuting(String command) {
if (command.equals("In")||command.equals("Out")) {
ImagePlus cimp = WindowManager.getCurrentImage();
if (cimp==null) return command;
if (cimp==imp) {
ImageCanvas ic = imp.getCanvas();
if (ic==null) return null;
int x = ic.screenX(crossLoc.x);
int y = ic.screenY(crossLoc.y);
if (command.equals("In")) {
ic.zoomIn(x, y);
if (ic.getMagnification()<=1.0) imp.repaintWindow();
} else {
ic.zoomOut(x, y);
if (ic.getMagnification()<1.0) imp.repaintWindow();
}
xyX=crossLoc.x; xyY=crossLoc.y;
update();
return null;
} else if (cimp==xz_image || cimp==yz_image) {
syncZoom = false;
return command;
} else
return command;
} else if (command.equals("Flip Vertically")&& xz_image!=null) {
if (xz_image==WindowManager.getCurrentImage()) {
flipXZ = !flipXZ;
update();
return null;
} else
return command;
} else
return command;
}
public void windowActivated(WindowEvent e) {
arrangeWindows(sticky);
}
public void windowClosed(WindowEvent e) {
}
public void windowClosing(WindowEvent e) {
if (!done)
dispose();
}
public void windowDeactivated(WindowEvent e) {
}
public void windowDeiconified(WindowEvent e) {
arrangeWindows(sticky);
}
public void windowIconified(WindowEvent e) {
}
public void windowOpened(WindowEvent e) {
}
public void adjustmentValueChanged(AdjustmentEvent e) {
update();
}
public void mouseWheelMoved(MouseWheelEvent e) {
if (e.getSource().equals(xz_image.getWindow())) {
crossLoc.y += e.getWheelRotation();
} else if (e.getSource().equals(yz_image.getWindow())) {
crossLoc.x += e.getWheelRotation();
}
update();
}
public void focusGained(FocusEvent e) {
ImageCanvas ic = imp.getCanvas();
if (ic!=null) canvas.requestFocus();
arrangeWindows(sticky);
}
public void focusLost(FocusEvent e) {
arrangeWindows(sticky);
}
public static ImagePlus getImage() {
if (instance!=null)
return instance.imp;
else
return null;
}
public static int getImageID() {
ImagePlus img = getImage();
return img!=null?img.getID():0;
}
public static void stop() {
if (instance!=null)
instance.dispose();
}
public static synchronized boolean isOrthoViewsImage(ImagePlus imp) {
if (imp==null || instance==null)
return false;
else
return imp==instance.imp || imp==instance.xz_image || imp==instance.yz_image;
}
public static Orthogonal_Views getInstance() {
return instance;
}
public int[] getCrossLoc() {
int[] loc = new int[3];
loc[0] = crossLoc.x;
loc[1] = crossLoc.y;
loc[2] = imp.getSlice()-1;
return loc;
}
public void setCrossLoc(int x, int y, int z) {
crossLoc.setLocation(x, y);
int slice = z+1;
if (slice!=imp.getSlice()) {
if (hyperstack)
imp.setPosition(imp.getChannel(), slice, imp.getFrame());
else
imp.setSlice(slice);
sliceSet = true;
}
while (!initialized) {
IJ.wait(10);
}
update();
}
public ImagePlus getXZImage(){
return xz_image;
}
public ImagePlus getYZImage(){
return yz_image;
}
public void run() {
while (!done) {
synchronized(this) {
try {wait();}
catch(InterruptedException e) {}
}
if (!done)
exec();
}
}
}
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